此页面上的某些内容不提供英文版本,默认显示为当前可用的语言。

Chemical phosphorylation reagent

目录号 数量 价格 提前通知时间
21560
/ 置于6mL带隔垫小瓶
250 mg $110 20 天
41560
/ 置于6mL带隔垫小瓶
1 g $325 现货
61560
/ 置于20mL带隔垫小瓶
5 g $875 现货
Found better price? Let us know and we will propose the way forward!

Chemical phosphorylation reagent for the synthesis of 5’-phosphorylated oligonucleotides. The reagent contains a DMT group in its structure, which allows oligonucleotide purification on C18-cartridges or by reversed-phase chromatography. To yield 5’-phosphorylated oligonucleotide, remove DMT group and then deprotect the phosphate group with diluted ammonium solution (0.1 M). Deprotection of the phosphate group is rapid and efficient even under mild basic conditions, so this reagent can be used for DMT-Off synthesis, for example for RNA synthesis, or with dye phosphoramidites that require mild deprotection conditions.

Usage

Diluent: anhydrous acetonitrile. Add the diluent to the recommended concentration (0.1 M) and wait until reagent dissolution is complete while mixing periodically. This reagent is viscous amorphous, and it may take up to 10 minutes to dissolve it. Store the diluted reagent in anhydrous conditions for not more than 24 hours.

Coupling: 6 minutes

Deprotection:

  1. DMT group is removed during synthesis: to yield the 5’-terminal phosphate, deprotect under standard conditions using ammonium hydroxide.
  2. DMT-ON, cartridge purification: standard cartridge purification conditions. After elution from the cartridge, deprotect the phosphate group by adding an equivalent volume of 25% aqueous ammonium solution to oligonucleotide solution, incubate for 15 minutes at room temperature, and dry the oligonucleotide down.
  3. DMT-ON, purification by HPLC: purify 5’-DMT-oligonucleotide by reversed-phase HPLC. To remove DMT group, redissolve oligonucleotide in 80% acetic acid and incubate for 30 minutes at room temperature. Dry the oligonucleotide down, add 10% aqueous ammonium and incubate for 15 minutes to cleave the remaining fragment of the protecting group.
  4. DMT-OFF: to yield the 5’-phosphate, use standard deprotection conditions after synthesis.

顾客还购买了这个产品

炔酰胺、羟基脯氨醇 (Alkyne amidite, hydroxyprolinol)

用于将乙炔基团掺入寡聚物中的乙炔酰胺。

Azide-PEG3-iodide

Azide-PEG3-iodide is a bifunctional linker with a highly reactive alkylative halogen functional group and an azido group for click chemistry or Staudinger ligation.

General properties

Appearance: colorless semisolid
Molecular weight: 722.8
CAS number: 171285-25-9
Molecular formula: C39H51N2O9P
IUPAC name: Propanedioic acid, 2-​[[bis(4-​methoxyphenyl)​phenylmethoxy]​methyl]​-​2-​[[[[bis(1-​methylethyl)​amino]​(2-​cyanoethoxy)​phosphino]​oxy]​methyl]​-​, 1,​3-​diethyl ester
Quality control: NMR 1H,31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
安全数据表(MSDS): 下载
产品规格

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: recommended coupling time 6 min
Deprotection conditions: identical to protected nucleobases
您的商品已添加. 查看您的 购物车 或者 继续结账
商品数量不正确.