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SIMA-dT phosphoramidite, 6-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2950-100mg | 100 mg | $180 | 现货 | |
| 2950-250mg | 250 mg | $390 | 现货 | |
| 2950-1g | 1 g |
$1120
|
现货 | |
| 2950-5g | 5 g |
$4520
|
21 天 | |
| 2950-10g | 10 g |
$8650
|
现货 |
SIMA-dT phosphoramidite is used to introduce SIMA in the sequence during oligonucleotide synthesis, usually as a substitute for the native dT linkage.
SIMA is known to be much more stable than HEX in basic media thus deprotection in harsh conditions using ammonium hydroxide (up to 6-8 hours at 55 °C) is possible as well as AMA at room temperature or at 65 °C.
Recommendations for using the reagent:
Coupling: 6 minutes coupling time recommended.
Deprotection: standard method recommended, can be deprotected with AMA (1:1 mixture of concentrated aqueous ammonium hydroxide / 40% aqueous methylamine).
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Chemical phosphorylation reagent
Reagent for the synthesis of 5’-phosphorylated oligonucleotides.SIMA 亚磷酰胺，6-异构体
一种亚磷酰胺，用于合成 SIMA 标记的寡核苷酸。SIMA 是一更稳定的HEX类似物。
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FAM phosphoramidite, 6-isomer (hydroxyprolinol)
Non-nucleoside phosphoramidite reagent suitable for labeling of oligonucleotides with fluorescein.
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General properties
| Appearance: | white powder |
| Molecular weight: | 1646.67 |
| Molecular formula: | C91H95Cl2N6O17P |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 531 |
| ε, L⋅mol−1⋅cm−1: | 92300 |
| Emission maximum, nm: | 555 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.57 |
| CF280: | 0.18 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
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