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CytoTracer Orange CMTMR

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CytoTracer Orange CMTMR is an orange fluorescent dye for labeling and long-term tracing of living cells. In working concentrations, the dye has little cytotoxicity and minimal effects on the proliferative ability or biology of the cell. It can be used to analyze cell proliferation, viability, localization, and motility for in vivo and in vitro assays.

CytoTracer Orange CMTMR is a cell-permeant stain that converts into cell-impermeant reaction products inside the cell. During proliferation, the label is transferred to daughter cells, but not adjacent cells in the population, and labeled cells keep the fluorescence for at least 72 hours or through three to six cell generations.

CytoTracer Orange CMTMR contains a chloromethyl group that reacts with thiol groups of internal cell components, utilizing a glutathione S-transferase-mediated reaction. This allows the stain to remain in cells after fixation and permeabilization steps and be used for subsequent immunofluorescence applications.

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DMSO (dimethyl sulfoxide), labeling grade

Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.

Fluo-4 AM, green fluorescent calcium indicator

Fluo-4 AM is a cell-permeable calcium ion indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon calcium ion binding. Fluo-4 AM is well suited for fluorometric and imaging applications.

General properties

Appearance: red solid
Molecular weight: 554.0
CAS number: 323192-14-9
Molecular formula: C32H28ClN3O4
IUPAC name: 9-[2-carboxy-4(or 5)-[[4-(chloromethyl)benzoyl]amino]phenyl]-3,6-bis(dimethylamino)-xanthylium, inner salt
Quality control: NMR 1H and HPLC-MS (85+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid exposure to light.
安全数据表(MSDS): 下载
产品规格

Spectral properties

Excitation/absorption maximum, nm: 540
Emission maximum, nm: 565
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