此页面上的某些内容不提供英文版本,默认显示为当前可用的语言。

5-Ethynyl-uridine (EU)

目录号 数量 价格 提前通知时间
2439-25mg 25 mg $137 现货
2439-100mg 100 mg $487 现货
2439-500mg 500 mg $950 现货
Found better price? Let us know and we will propose the way forward!

5-Ethynyl-uridine (EU) is a commonly used molecular biology reagent for RNA synthesis studies.

EU is readily taken up by living cells and phosphorylated through the pyrimidine salvage pathway. Generated 5-ethynyluridine-5’-triphosphate is incorporated by RNA polymerases I, II, and III into de novo RNA instead of uridine, but not into DNA.

EU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via click chemistry following fluorescent visualization. Alkyne group attached at the 5-position of uridine in modified RNA reacts with dye or biotin azides via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC).

Labeled RNA can be detected with different methods, e.g. fluorescent microscopy or flow cytometry, which allows estimating transcriptional levels in the cells.

5-Ethynyl-uridine has an advantage over its analog, 5-bromo-uridine, because azide-containing dyes are very small in size and exhibit better membrane permeability compared to antibodies used for the detection of 5-bromo-uridine. Thus, the click chemistry approach allows whole-mount staining of large samples like organs or tissue fragments.

顾客还购买了这个产品

AF 488 叠氮化物 (AF 488 azide)

AF 488 叠氮化物是一种发射在绿色光谱范围内的荧光团。它与炔烃在铜催化的条件下发生环加成反应。

Ac4GalNAl(N-(4-戊酰基)-半乳糖胺四酰化) [Ac4GalNAl (N-(4-pentynoyl)-galactosamine-tetraacylated)]

一种炔烃功能化的单糖用于代谢标记,研究标记蛋白质的细胞运输和蛋白质组学分析。
将此产品添加到购物车中
并获得免费的快递服务

AF 343(香豆素)-X-NHS 酯 (AF 343 (Coumarin)-X-NHS ester)

AF 343(香豆素)是一种蓝光发射染料,可用作荧光素 (FAM) 的 FRET 供体。该结构的特点是香豆素 343 荧光团和官能团之间有一个连接体。它提供了更好的溶解性以及荧光团和官能团之间的空间分离。

General properties

Appearance: off white solid
Molecular weight: 268.22
CAS number: 69075-42-9
Molecular formula: C11H12N2O6
IUPAC name: 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione
Solubility: good in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
安全数据表(MSDS): 下载
产品规格
您的商品已添加. 查看您的 购物车 或者 继续结账
商品数量不正确.