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JOE phosphoramidite, 5-isomer
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
C1160 | 100 mg | – | 现货 | |
D1160 | 250 mg | $165 | 现货 | |
F1160 | 1 g | $575 | 现货 | |
G1160 | 5 g | $2230 | 现货 | |
H1160 | 10 g | $3300 | 现货 |
JOE is a xanthene dye, fluorescein derivative possessing two chloro- and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.
The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains a pure isomer of 5-JOE dye.
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Cyanine 5.5叠氮化物 (Cyanine5.5 azide)
用于点击化学的远红/近红外荧光 Cyanine5.5 染料衍生物。R6G azide, 5-isomer
Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of click chemistry.Copper(II)-TBTA complex
A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.General properties
Appearance: | colorless solid |
Molecular weight: | 972.88 |
Molecular formula: | C48H60N3Cl2O12P |
Solubility: | good in DCM, acetonitrile |
Quality control: | NMR 1H, 31P, HPLC-MS (95%), functional testing |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 533 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 554 |
Fluorescence quantum yield: | 0.61 |
CF260: | 0.36 |
CF280: | 0.28 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Cleavage conditions: | ammonia, 2 h at room temperature |
Deprotection conditions: | identical to protected nucleobases |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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