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JOE phosphoramidite, 5-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| C1160 | 100 mg | – | 现货 | |
| D1160 | 250 mg |
$165
$66
|
现货 | |
| F1160 | 1 g |
$575
$229
|
现货 | |
| G1160 | 5 g |
$2230
$892
|
现货 | |
| H1160 | 10 g |
$3300
$1320
|
现货 |
JOE is a xanthene dye, fluorescein derivative possessing two chloro- and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.
The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains a pure isomer of 5-JOE dye.
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FAM-dT亚磷酰胺
亚磷酰胺用于在合成序列中间，用荧光素 (FAM) 标记寡核苷酸。
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JOE 叠氮化物，5-异构体 (JOE azide, 5-isomer)
JOE 是一种氯代荧光素染料，常用于寡核苷酸的 HEX 通道。它的疏水性比 HEX 弱。是一种叠氮衍生物，适用于点击化学。HEX 亚磷酰胺，6-异构体 (HEX phosphoramidite, 6-isomer)
HEX 亚磷酰胺用于合成 5' 标记寡核苷酸的。
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General properties
| Appearance: | colorless solid |
| Molecular weight: | 972.88 |
| Molecular formula: | C48H60N3Cl2O12P |
| Solubility: | good in DCM, acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%), functional testing |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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