DusQ 1 phosphoramidite
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 1470-100mg | 100 mg | $290 | 现货 | |
| 1470-500mg | 500 mg |
$990
|
现货 | |
| 1470-1g | 1 g |
$1690
|
现货 | |
| 1470-10g | 10 g | 请查询 | 现货 |
DusQ 1 phosphoramidite is a true dark quencher with broad absorption curve which covers the visible spectrum with maximum in green to yellow region. It is used for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5'-quencher DusQ 1 and other FRET applications for multiplexing assays. Contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges.
DusQ 1 has a quenching range (QR) 480 - 580 nm to construct efficiently quenched qPCR probes paired with all common reporter dyes such as FAM, TET, JOE, HEX and Cyanine3 with high quenching efficiency, and form completely non-fluorescent complexes.
Usage
Coupling: 6 minutes coupling time recommended.
Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).
Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.
顾客还购买了这个产品
DusQ1 dT 亚磷酰胺 (DusQ 1 dT phosphoramidite)
用于寡核苷酸DusQ1 dT 标记的亚磷酰胺。
将此产品添加到购物车中并获得免费的快递服务
FAM 叠氮化物，6-异构体 (FAM azide, 6-isomer)
FAM（荧光素）叠氮化物，6-异构体，是一种常用于点击化学的荧光染料叠氮化物。ROX NHS 酯，6-异构体
ROX（罗丹明X，R101）NHS-酯的纯 6-异构体，用于胺标记。
将此产品添加到购物车中并获得免费的快递服务
General properties
| Appearance: | black powder |
| Molecular weight: | 995.11 |
| CAS number: | 374591-94-3 |
| Molecular formula: | C55H63N8O8P |
| IUPAC name: | Phosphoramidous acid, bis(1-methylethyl)-, 2-[[2-[bis(4-methoxyphenyl)phenylmethoxy]ethyl][4-[[2-methoxy-5-methyl-4-[(4-methyl-2-nitrophenyl)azo]phenyl]azo]phenyl]amino]ethyl 2-cyanoethyl ester (9CI) |
| Solubility: | good in acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 522 |
| ε, L⋅mol−1⋅cm−1: | 27300 |
Oligo synthesis details
| Diluent: | 50% DCM in acetonitrile |
| Coupling conditions: | 6 min coupling time; 3 min oxidation time |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
您的购物车是空的
$ 
