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LUCS® 9, green fluorescent nucleic acid stain

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2M010 100 uL, 5 mM/DMSO $179 20 天
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LUCS 9 is a cell-permeant nucleic acid stain that exhibits green fluorescence upon binding to nucleic acids. The stain has a high fluorescent yield and a structure identical to SYTO™9 stain.

LUCS 9 is used to stain both DNA and RNA in live and dead eukaryotic cells as well as Gram-positive and Gram-negative bacteria. The dye is excited by the blue laser at 485 nm. Its fluorescence emission is detected in the fluorescein channel with a peak at 495 nm when bound to DNA and 501 nm when bound to RNA.

The dye can be used in simultaneous labeling with cell-impermeant nuclear markers, such as YoDi-3, propidium iodide, or 7-AAD to evaluate cell viability using fluorescence microscopy and flow cytometry.

LUCS 9 is especially useful as a counterstain for bacterial assays due to its ability to stain both live and dead Gram-negative and Gram-positive bacteria.

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General properties

Appearance: orange solution
Molecular weight: 534.44
Molecular formula: C28H27IN2O
Solubility: miscible with water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
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产品规格

Spectral properties

Excitation/absorption maximum, nm: 485
Emission maximum, nm: 498 (DNA complex)
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