Copper(II)-THPTA catalytic buffer, 1.5x
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
B5150 | 1 mL | $30 | 现货 | |
K5150 | 10 mL | $120 | 现货 | |
S5150 | 50 mL |
$490
|
现货 |
Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5× buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.
Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine
顾客还购买了这个产品
VIC azide, 6-isomer
VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.YODi-3, red fluorescent nucleic acid stain
YODi-3 is a cell-impermeant red fluorescent nucleic acid stain. YODi-3 is chemically equivalent to YOYO®-3.General properties
Storage conditions: | Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
安全数据表(MSDS): | 下载 |
产品规格 |
