Lumizol Reagent
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
91415 | 50 mL | $105 | 现货 |
Lumizol reagent includes phenol, guanidine isothiocyanate and other components which makes the high-quality RNA extraction possible. Such components as phenol and guanidine isothiocyanate provide cell lysis and inhibit ribonucleases effectively. Thus the RNA is preserved intact.
After samples homogenization followed by the addition of chloroform the phase separation occurs: an upper, aqueous phase, the middle interphase and lower organic phase. RNA in the upper aqueous phase is then collected by precipitation with isopropanol, while DNA and proteins might be collected from the interphase and organic phase. DNA isolation from interphase and lower organic phase can be performed using ethanol solution and for proteins by precipitation with isopropanol from supernatant.
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Azidomethyldeoxyuridine (AmdU) is an azide-contained nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain-promoted click reaction.sulfo-Cyanine3 tyramide
A tyramide derivative of sulfo-Cyanine3, an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
Appearance: | yellow-orange solution |
Quality control: | functional testing |
Storage conditions: | Storage and transportation: 12 months after receival at room temperature. |
安全数据表(MSDS): | 下载 |
产品规格 |
产品引用
- Borisenko, I.; Predeus, A.; Lavrov, A.; Ereskovsky, A. First draft genome assembly and characterization of sponge Halisarca dujardinii reveals key components of basement membrane and broad repertoire of aggregation factors. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.06.578935
