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Lumizol Reagent

Nucleic acid quantification DNA isolation RNA isolation
目录号 数量 价格 提前通知时间
91415 50 mL $105 现货
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Lumizol reagent includes phenol, guanidine isothiocyanate and other components which makes the high-quality RNA extraction possible. Such components as phenol and guanidine isothiocyanate provide cell lysis and inhibit ribonucleases effectively. Thus the RNA is preserved intact.

After samples homogenization followed by the addition of chloroform the phase separation occurs: an upper, aqueous phase, the middle interphase and lower organic phase. RNA in the upper aqueous phase is then collected by precipitation with isopropanol, while DNA and proteins might be collected from the interphase and organic phase. DNA isolation from interphase and lower organic phase can be performed using ethanol solution and for proteins by precipitation with isopropanol from supernatant.

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General properties

Appearance: yellow-orange solution
Quality control: functional testing
Storage conditions: Storage and transportation: 12 months after receival at room temperature.
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产品引用

  1. Borisenko, I.; Predeus, A.; Lavrov, A.; Ereskovsky, A. First draft genome assembly and characterization of spongeHalisarca dujardiniireveals key components of basement membrane and broad repertoire of aggregation factors. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.06.578935
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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