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Lumizol Reagent

目录号 数量 价格 提前通知时间
91415 50 mL $105 现货
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Lumizol reagent includes phenol, guanidine isothiocyanate and other components which makes the high-quality RNA extraction possible. Such components as phenol and guanidine isothiocyanate provide cell lysis and inhibit ribonucleases effectively. Thus the RNA is preserved intact.

After samples homogenization followed by the addition of chloroform the phase separation occurs: an upper, aqueous phase, the middle interphase and lower organic phase. RNA in the upper aqueous phase is then collected by precipitation with isopropanol, while DNA and proteins might be collected from the interphase and organic phase. DNA isolation from interphase and lower organic phase can be performed using ethanol solution and for proteins by precipitation with isopropanol from supernatant.

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General properties

Appearance: yellow-orange solution
Quality control: functional testing
Storage conditions: Storage and transportation: 12 months after receival at room temperature.
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产品规格

产品引用

  1. Borisenko, I.; Predeus, A.; Lavrov, A.; Ereskovsky, A. First draft genome assembly and characterization of sponge Halisarca dujardinii reveals key components of basement membrane and broad repertoire of aggregation factors. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.06.578935
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