Universal CPG type II, 500A
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 3219-1g | 1 g |
$45
|
现货 | |
| 3219-10g | 10 g |
$410
|
现货 | |
| 3219-100g | 100 g |
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21 天 |
The Universal CPG type II, 500A is used for oligonucleotides synthesis to increase rate of dephosphorylation of the 3' end oligonucleotide during deblocking due to a rigid bicyclic molecule on the support.
The Universal CPG type II, 500A is suitable for use in harsh conditions and makes cleavage and deprotection with anhydrous ammonia gas-phase, ammonium hydroxide/methylamine (AMA) mixture or other basic reagents faster compared to universal Controlled Pore Glass (CPG) supports. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 120 bases. For oligos up to 120 bases universal support 1000 Å can be used.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 2 hours at 80
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FAM 叠氮化物，6-异构体 (FAM azide, 6-isomer)
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用于寡核苷酸合成的 TFA-氨基修饰剂 CPG。 500 Å，高负载可控孔径玻璃。VIC azide, 6-isomer
VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.General properties
| Appearance: | white powder |
| Quality control: | loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonium hydroxide 2 hours at 80 °C or AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 15 minutes at 65 °C |
| Deprotection conditions: | identical to protected nucleobases |
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