R6G phosphoramidite, 6-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| C5260 | 100 mg | – | 现货 | |
| D5260 | 250 mg | $190 | 现货 | |
| F5260 | 1 g |
$590
|
现货 | |
| G5260 | 5 g |
$1950
|
现货 | |
| H5260 | 10 g | 请查询 | 现货 |
R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.
R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.
R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.
Usage:
Coupling: 10 minutes.
Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.
Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.
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BDP TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.VIC phosphoramidite, 6-isomer
VIC is an asymmetrical xanthene dye with spectral properties similar to HEX and JOE. Useful as a label for qPCR probes.
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JOE 叠氮化物，5-异构体 (JOE azide, 5-isomer)
JOE 是一种氯代荧光素染料，常用于寡核苷酸的 HEX 通道。它的疏水性比 HEX 弱。是一种叠氮衍生物，适用于点击化学。General properties
| Appearance: | yellowish powder |
| Molecular weight: | 949.93 |
| CAS number: | 1355330-47-0 |
| Molecular formula: | C46H54F6N5O8P |
| Solubility: | good in DCM, acetonitrile |
| Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 518 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.95 |
| CF260: | 0.18 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
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