R6G phosphoramidite, 6-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| C5260 | 100 mg | – | 现货 | |
| D5260 | 250 mg | $190 | 5 天 | |
| F5260 | 1 g |
$590
|
5 天 | |
| G5260 | 5 g |
$1950
|
现货 | |
| H5260 | 10 g | 请查询 | 现货 |
R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.
R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.
R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.
Usage:
Coupling: 10 minutes.
Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.
Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.
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Cyanine5亚磷酰胺 (Cyanine5 phosphoramidite)
Cyanine5 亚磷酰胺衍生物，用于寡核苷酸合成FAM 叠氮化物，6-异构体 (FAM azide, 6-isomer)
FAM（荧光素）叠氮化物，6-异构体，是一种常用于点击化学的荧光染料叠氮化物。BDP® TR azide
BDP TR is a red-emitting fluorophore for the ROX channel. This is an azide for copper-catalyzed and copper-free сlick chemistry conjugation.General properties
| Appearance: | yellowish powder |
| Molecular weight: | 949.93 |
| CAS number: | 1355330-47-0 |
| Molecular formula: | C46H54F6N5O8P |
| Solubility: | good in DCM, acetonitrile |
| Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 518 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.95 |
| CF260: | 0.18 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
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