ProteOrange® Protein Quantification Reagent, 500×
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 11210 | 100 uL | – | 现货 | |
| 41210 | 1 mL | $195 | 现货 |
ProteOrange® is a fluorescent protein-sensitive dye that exhibits almost no fluorescence in the free form but fluoresces upon binding to proteins. The excitation maximum of ProteOrange in the complex with protein is at ~485 nm, the emission maximum — at ~590 nm.
The unique structure of the ProteOrange reagent allows accurate detection of proteins in solutions with concentrations between 10 ng/mL and 10 µg/mL. This level of sensitivity is considerably better than that achieved with the BCA method, Bradford assay, Lowry assay, or absorption measurements at 280 nm. The dye shows minimal variability for different proteins.
The ProteOrange dye has a low tolerance to non-ionic detergents. It is not recommended for cell lysates containing Triton X-100 and sodium deoxycholate, but it can tolerate up to 0.01% SDS (in final concentration).
This reagent is a 500× concentrate of ProteOrange dye. This product is a component of ProteOrange Protein Quantification Kit.
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PreciseGreen® dsDNA 定量试剂，200× (PreciseGreen dsDNA Quantification Reagent, 200×)
PreciseGreen® 是一种染料，可用于在RNA、ssDNA 和其他杂质存在的情况下对dsDNA进行精确荧光定量。该染料在荧光素通道中表现出明亮的荧光，在pg/mLng/mL 范围内对 DNA 浓度提供线性响应。dsGreen® 用于实时定量PCR, 100× (dsGreen® for Real-Time PCR, 100×)
用于实时定量PCR 的 dsGreen® 储备液，100×BDP® FL胺 (BDP® FL amine)
BDP FL 染料的氨基衍生物，用于 FAM（荧光素）488 nm 通道的硼二吡咯亚甲基染料。General properties
| Appearance: | Orange solution |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 470 |
| Emission maximum, nm: | 570 |
产品引用
- Kladova, O.A.; Tyugashev, T.E.; Yakimov, D.V.; Mikushina, E.S.; Novopashina, D.S.; Kuznetsov, N.A.; Kuznetsova, A.A. The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. International Journal of Molecular Sciences, 2024, 25(8), 4182. doi: 10.3390/ijms25084182
- Grigorenko, V.G.; Krivitskaya, A.V.; Khrenova, M.G.; Rubtsova, M.Y..; Presnova, G.V.; Andreeva, I.P.; Serova, O.V.; Egorov, A.M. Saturation Mutagenesis and Molecular Modeling: The Impact of Methionine 182 Substitutions on the Stability of β-Lactamase TEM-1. International Journal of Molecular Sciences, 2024, 25(14), 7691. doi: 10.3390/ijms25147691
- Berditchevskii, G.M.; Vasina, L.V.; Ageev, S.V.; Meshcheriakov, A.A.; Galkin, M.A.; Ishmukhametov, R.R.; Nashchekin, A.V.; Kirilenko, D.A.; Petrov, A.V.; Martynova, S.D.; Semenov, K.N.; Sharoyko, V.V. A comprehensive study of biocompatibility of detonation nanodiamonds. Journal of Molecular Liquids, 2021, 332, 115763. doi: 10.1016/j.molliq.2021.115763
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