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DiR, lipophilic tracer

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DiR is a near-infrared fluorescent carbocyanine dye for cell membrane labeling in vivo and in vitro. DiR diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. The dye is weakly fluorescent until incorporated into membranes.

DiR can be used with other tracers in multi-color studies, such as DiI or DiO and for NIR imaging.

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General properties

Appearance: violet-green sticky solid
Molecular weight: 1013.42
CAS number: 100068-60-8
Molecular formula: C63H101IN2
Solubility: DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
安全数据表(MSDS): 下载
产品规格

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 254000
Emission maximum, nm: 775
Fluorescence quantum yield: 0.25
CF260: 0.08
CF280: 0.08
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